Susanne Rafelski, Allen Institute for Cell Science, Seattle
When
Where
Abstract: Single-cell imaging and analytics are advancing at a breakneck pace via the collection of large-scale, systematic cell image datasets and the application of quantitative image-based data science methods. This is, therefore, a key moment in the arc of biological research to develop methods and analyses that integrate the spatiotemporal observables of the physical structure and organization of the cell with molecular observables, and to update the concept of a holistic cell state. We propose a conceptual framework for holistic cell states and state transitions that is practical and useful and enables integrative analyses and modeling across many data types. We are exploring appropriate experimental, computational, and theoretical frameworks to test hypotheses within the holistic cell state framework. These include using model systems for state transitions such as human induced pluripotent stem cell (hiPSC)-based EMT during early differentiation and hiPSC-derived endothelial cell response to changes in shear stress. We are also developing computational approaches to quantify cell organization and cell function within this framework in undifferentiated hiPSCs. Our efforts necessarily include developing image analysis, visual analysis, data analysis, and simulation tools, which we release openly and, in some cases, develop further for greater community accessibility and useability.
3:00 PM in PAS 201 / Zoom Meeting https://arizona.zoom.us/j/81283840289
Refreshments in PAS 218, 2:30PM
Additional Information:
Speaker presents also at the
70th Annual Meeting of the Radiation Research Society at the Westin La Paloma,
https://web.cvent.com/event/f628c567-73e4-4fee-a58b-1e9b6b0c1981/summary
Presidential Symposium: Wednesday, Sept 18 3:15-5:15 PM
Topic: Mechanisms of radiation response
Restricted to registered participants